HPLC vs. Mass Spectrometry: What Each Test Measures

Two analytical techniques appear on most peptide COAs: high-performance liquid chromatography (HPLC) and mass spectrometry (MS). They answer different questions.

HPLC: how pure?

HPLC separates the components of a sample, allowing the relative purity of the main peak to be quantified.

Mass spectrometry: is it the right molecule?

MS measures molecular mass, confirming the identity of the compound against its expected value.

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